Hybrid High Resolution MSn: LTQ FT Ultra

The LTQ FT Ultra combines the most advanced Ion Trap and Fourier Transform Ion Cyclotron Resonance technologies into a single instrument with unprecedented analytical power and versatility. Ultra-high resolution and sensitivity coupled with sub ppm mass accuracy, and MSⁿ are universally available for the toughest applications demanding rigorous structural characterization.

The Ultra Cell - The Heart of the New FTICR

The Thermo Scientific LTQ FT Ultra mass spectrometer is the next generation hybrid FTICR featuring a novel ICR cell design, the Ultra Cell. This cell design produces enhanced sensitivity and greater dynamic range. The linear excitation characteristics of the cell allow larger cyclotron radii which significantly reduces space charge effects. These performance gains are achieved without the costs and difficulties associated with a higher field magnet.

• Attomole sensitivity
• Widest dynamic range ( > 4,000)
• ppb mass accuracy
• Resolution > 750,000

The new LTQ FT Ultra is built on the proven Thermo Scientific linear ion trap and FTICR platform, featuring:

• Automatic Gain Control - concentration independent ppb mass accuracy with space charge control
• Parrallel Acquisition - unmatched data dependant acquisition speed
• ECD and IRMPD - detailed characterization of peptides and proteins
• Fast Scan Rate with High Resolution - compatible with LC timescale
• Automated Optimization and Acquisition - simplified operation

ATTOMOLE SENSITIVITY
For identification of low abundance peptides

50 attomole on column On-line nano-LC-MS/MS of a 5 protein mix digest (50 attomole each on column, 30 min gradient). Data analysed with BioWorks 3.3. All proteins found with at least 2 peptides, high Xcorr and high peptide probability.

Maintain mass accuracy over a
WIDER DYNAMIC RANGE

Concentration independent mass accuracy in infusion or under LC conditions. Detect and correctly identify low abundant co-eluting species.

Single spectrum of a nano-LC-MS/MS run of a Yeast Enolase digest

UNMATCHED MASS ACCURACY

Eliminates false positive identifications in bottom-up proteomics. Unambigous identification of unknown analytes with on-line LC-MS/MS at any concentration.

Analysis of peptide MRFA, m/z 524

ULTRA HIGH RESOLUTION
for analysis of complex mixtures

Ultra high resolution available on demand with a single mouse click. Ultra high resolution is essential for the analysis of complex samples such as crude oil or Disolved Organic Matter (DOM).

Measured versus simulated 2nd isotope peak of peptide MRFA, at m/z 526.265